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US 9,588,123 B2Granted
Compositions and methods for in vitro diagnostic tests including zwitterionic solubilization reagent
AssigneeSurmodics Ivd, Inc.
InventorSean Lundquist
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AssigneeSurmodics Ivd, Inc.
InventorSean Lundquist
Estimating the term…
The disclosure is directed to compositions, kits, and methods for performing colorimetric analysis. A composition for a colorimetric assay can be prepared that includes an aromatic amine chromogenic substrate and a zwitterionic solubilization reagent that is N,N,N-trimethylglycine (betaine). The zwitterionic solubilization reagent is highly useful as a non-encapsulating solubilization reagent and provided faster kinetics and increased dye content of an aromatic amine chromogenic substrate in an enzymatic reaction. In turn, this can result in improvements in detection of an analyte in a sample and also can increase the sample throughput. The aromatic amine chromogenic substrate and N,N,N-trimethylglycine solubilization reagent can be used in assays such as ELISAs in order to provide a more accurate and faster detection of analytes in a biological sample.
An aqueous composition for colorimetric analysis comprising a benzidine chromogenic substrate and N,N,N-trimethylglycine, wherein the benzidine chromogenic substrate is 3,3′,5,5′ tetramethylbenzidine (3,3′,5,5′ TMB) wherein the N,N,N-trimethylglycine is present in the composition in the range of 0.25 M to 1 M, wherein the composition has a pH in the range of 3 to 4, wherein the N,N,N-trimethylglycine and 3,3′,5,5′ TMB are present in the composition in a molar ratio in the range of 20:1 to 1,000:1, and wherein the N,N,N-trimethylglycine improves the solubility of the 3,3′,5,5′ TMB in the aqueous composition.
Dependencies are inferred from the claim text.