# US 9,588,107 B2 · Tool for studying endothelial haematopoietic transition (EHT) and epithelial-mesenchymal transition (EMT) associated events

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- Number: US 9,588,107 B2
- Document ID: US9588107B2
- Status: Granted
- Kind: B2
- Application number: 13/383,973
- Filed: 2010-07-14
- Published: 2012-07-12
- Granted: 2017-03-07
- Earliest claimed: 2010-07-14
- Assignees: Azelead
- Applicants: Karima Kissa-Marin; Philippe Herbomel
- Inventors: Karima Kissa-Marin; Philippe Herbomel
- Web page: https://patentgrep.com/patent/US9588107B2

## CPC

- G01N 33/5088: {of vertebrates}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The present invention relates to the field of endothelial haematopoietic transition (EHT) and epithelial-mesenchymal transition (EMT) and more particularly relates to the use of fish embryo or larva as a model for the study of EHT and EMT.

## Claims

### Claim 1

A method of screening for a candidate compound that modulates the endothelial haematopoietic transition (EHT) mechanism, comprising the steps of:
introducing candidate compound in a water tank containing a transgenic zebrafish embryo or larva, said transgenic zebrafish embryo or larva expressing a marker selected from the group consisting of CD41, runx1, cmyb, KDR, kdrl, Fli, Fli-1, Lmo2, ephrinB2 and Tie-2, and said marker being coupled to a reporter gene selected from the group consisting of green fluorescent protein (GFP) and discosoma red protein (Dsred), and
detecting the apparition of fluorescent transgenic cells in the tail of the zebrafish embryo or larva to determine the capacity of said candidate compound to inhibit the transition of aortic endothelial cells into haematopoietic stem cells.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the transgenic zebrafish embryo or larva expresses the marker CD41 coupled to the reporter gene GFP.