Escherichia coli capable of producing 3-amino-4-hydroxybenzoic acid
AssigneeAjinomoto Co., Inc.
InventorsFumi Shozui, Yoshinori Tajima
Life of this patent
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Abstract
The present invention provides a method for conveniently and inexpensively producing an amino-hydroxybenzoic acid-type compound such as 3-amino-4-hydroxybenzoic acid by utilizing Escherichia coli that is a bacterium commonly used for a production process by biosynthesis. Specifically, the present invention provides an Escherichia coli having an ability to produce 3-amino-4-hydroxybenzoic acid, which is modified to reduce an activity of N-hydroxyarylamine O-acetyltransferase (NhoA); a method for producing a 3-amino-4-hydroxybenzoic acid-type compound using such Escherichia coli; and the like.
Claims11
A method for producing a 3-amino-4-hydroxybenzoic acid or a salt thereof, comprising a step of culturing an Escherichia coli bacterium having an ability to produce 3-amino-4-hydroxybenzoic acid which has been genetically modified to express DNA which encodes an enzyme to increase the activity of an enzyme, wherein said enzyme is capable of catalyzing formation of 3-amino-4-hydroxybenzoic acid from dihydroxyacetone phosphate and aspartate semialdehyde as compared to a non-modified Escherichia coli bacterium, and wherein said enzyme is GriI and GriH;
- and wherein the Escherichia coli bacterium also has been modified to inactivate an N-hydroxyarylamine O-acetyltransferase (NhoA) as compared to a non-modified Escherichia coli bacterium by mutating or deleting an nhoA gene on the chromosome of the Escherichia coli bacterium.
Dependencies are inferred from the claim text.