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AssigneesUniversite De Rouen, L'institut Francais De Recherche Pour L'exploitation De La Mer (Ifremer), Algenics
InventorsMuriel Bardor, Romain Louvet, Bruno Saint-Jean, Carole Burel, Bérangère Baiet, Jean-Paul Cadoret, Patrice Lerouge, Rémy Michel, Aude Carlier
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Transformed Phaeodactylum tricornutum including a nucleic acid sequence operatively linked to a promoter, wherein the nucleic acid sequence encodes an N-acetylglucosaminyltransferase I and/or an α-Mannosidase II and wherein at least one β-N-acetylglucosaminidase of the transformed Phaeodactylum tricornutum has been inactivated. A method for producing a glycosylated polypeptide includes the steps of (i) culturing a transformed P. tricornutum as defined previously and (ii) purifying the polypeptide that is expressed and glycosylated in the transformed P. Tricornutum. The use of such a transformed P. tricornutum for producing a glycosylated polypeptide is also described.
A transformed Phaeodactylum tricornutum, whose N-glycosylation pathway has been modified by inactivation of terminal N-acetylglucosamine residue removal activity of at least one β-N-acetylglucosaminidase.
Dependencies are inferred from the claim text.