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US 9,587,241 B2Granted
Biosynthesis of human milk oligosaccharides in engineered bacteria
AssigneeGlycosyn LLC
InventorsMassimo Merighi, John M. McCoy, Matthew Ian Heidtman
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Abstract
The invention provides compositions and methods for engineering bacteria to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection.
Claims28
An isolated E. coli bacterium comprising
- (i) a deletion or functional inactivation of an endogenous β-galactosidase gene;
- (ii) an exogenous functional β-galactosidase gene comprising a detectable level of β-galactosidase activity that is reduced compared to that of a wild-type E. coli bacterium, wherein the level of β-galactosidase activity comprises between 0.05 and 200 units;
- (iii) an inactivating mutation in a colanic acid synthesis gene;
- and
- (iv) an exogenous lactose-accepting fucosyltransferase gene.
2 more elements
Dependencies are inferred from the claim text.