Loading patent…
AssigneeVirginia Tech Intellectual Properties, Inc.
InventorsXiang-Jin Meng, Nathan M. Beach, Sheela Ramamoorthy
Estimating the term…
The present invention provides a novel chimeric porcine circovirus infectious DNA clone and live attenuated chimeric virus with the PCV2, preferably of subtype PCV2b, capsid gene integrated into a non-pathogenic PCV1 virus genome. In a particular embodiment, the PCV2 capids gene is of subtype PCV2b, the predominant subtype circulating in pigs worldwide. The attenuated chimeric virus, designated PCV1-2b, effectively protects pigs from PCV2b challenges, and can be used as a live vaccine, as well as an inactivated (killed) vaccine, that provides protection and cross protection against PCV2b and PCV2a subtypes infection. The live attenuated vaccine of the present invention is also effective protecting pigs from porcine circovirus-associated disease (PCVAD).
A chimeric PCV1-2b virus comprising a recombinant PCV1 that encodes the capsid protein of PCV2b subtype in place of the capsid protein of PCV1, wherein the capsid protein of the PCV2b subtype is encoded by the ORF2 capsid gene of a wild-type PCV2b strain having the full-length genomic nucleotide sequence set forth in SEQ ID NO:
A viral vaccine comprising a physiologically acceptable carrier and an immunogenic amount of a chimeric PCV1-2b virus which comprises a recombinant PCV1 that encodes the capsid protein of PCV2b subtype in place of the capsid protein of PCV1, wherein the capsid protein of the PCV2b subtype is encoded by the ORF2 capsid gene of a wild-type PCV2b strain having the full-length genomic nucleotide sequence set forth in SEQ ID NO:
Dependencies are inferred from the claim text.