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US 9,582,877 B2Granted
Methods and systems for digitally counting features on arrays
AssigneeCellular Research, Inc.
InventorsGlenn Fu, Roger Rudoff, David Stern, George T. Wu, Stephen P. A. Fodor, Geoffrey Facer
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Abstract
Methods, systems and platforms for digital imaging of multiple regions of an array, and detection and counting of the labeled features thereon, are described.
Claims44
An array reader system comprising
- a computer system comprising a digital processor configured to execute computer-executable instructions to at least:
- (a) acquiring image data of a region of an array, wherein the array comprises a plurality of stochastically labeled and non-labeled features, and wherein the features comprise oligonucleotide probes;
- (b) measuring a signal intensity and a local background intensity for one of the plurality of stochastically labeled and non-labeled features based on the acquired image data;
- (c) calculating a local background corrected signal intensity for the one of the plurality of stochastically labeled and non-labeled features using the signal intensity and the local background intensity measured in (b);
- (d) transforming the local background corrected signal intensity calculated in (c) for the one of the plurality of stochastically labeled and non-labeled features to determine the number of stochastically labeled and non-labeled features in the of the array;
- and
- (e) calculating the absolute number of a target molecule based on the number of stochastically labeled and non-labeled features determined in (d) within the region of the array.
4 more elements
Dependencies are inferred from the claim text.