Holographic microscope, holographic image generation method, and method for acquiring data for holographic image

A method for generating a holographic tomographic image, comprising the steps of:

• illuminating an object with an illumination light;
• acquiring an off-axis hologram of an object light emitted from the object and an off-axis hologram of the illumination light using an off-axis spherical wave reference light;
• acquiring an off-axis hologram of the off-axis spherical wave reference light using an in-line spherical wave reference light;
• deriving a complex amplitude in-line hologram of the object light and a complex amplitude in-line hologram of the illumination light from the off-axis holograms of the object light and the illumination light using the off-axis hologram of the in-line spherical wave reference light, wherein the deriving of the complex amplitude in-line holograms is performed using lights of each wavelength generated by changing the wavelength of the illumination light, the off-axis spherical wave reference light, and the in-line spherical wave reference light to derive the complex amplitude in-line holograms for every wavelength;
• deriving, on a specific reconstruction plane, from each of the complex amplitude in-line holograms, a reconstructed light wave of the object light, a reconstructed light wave of the illumination light, and a phase component contained in the reconstructed light wave of the illumination light;
• deriving a phase adjusted reconstruction light wave by removing the phase component from the reconstructed light wave of the object light for every light of each wavelength, and then derives a tomographic hologram on the specific reconstruction plane by adding up the phase adjusted reconstruction light wave for the wavelength;
• and
• generating the tomographic image on the specific reconstruction plane from the tomographic hologram.

A data acquisition method for a holographic tomographic image, comprising the steps of:

• recording an object light, emitted from an object illuminated with an illumination light, in an off-axis hologram using an off-axis spherical wave reference light;
• recording the illumination light in an off-axis hologram using the off-axis spherical wave reference light;
• and
• recording the off-axis spherical wave reference light (R) in an off-axis hologram using an in-line spherical wave reference light, wherein
• the recordings of the illumination light, the off-axis spherical wave reference light, and the in-line spherical wave reference light are repeated with lights, which are the illumination light, the off-axis spherical wave reference light, and the in-line spherical wave reference light having changed wavelength, so that holograms for each wavelength are stored as data for a holographic tomographic image.

A data acquisition method for a holographic tomographic image, comprising the steps of:

• recording an object light, emitted from the object illuminated by an illumination light, in a hologram using a reference light;
• recording the illumination light in a hologram using the reference light;
• and
• recording the reference light in an off-axis hologram using an in-line reference light, wherein
• the recordings of the illumination light, the reference light, and the in-line reference light are repeated with lights, which are the illumination light, the reference light, and the in-line reference light having changed wavelength, so that holograms for each wavelength are stored as data for a holographic tomographic image.