Method for producing γ-glutamylvalylglycine or a salt thereof
AssigneeAjinomoto Co., Inc.
InventorsHiroyuki Nozaki, Isao Abe, Jun Takakura, Rie Takeshita, Hideyuki Suzuki, Shunichi Suzuki
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Abstract
A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.
Claims19
A method for producing γ-Glu-Val-Gly comprising reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase such that γ-Glu-Val-Gly is generated, wherein the γ-glutamyltransferase comprises a large subunit and a small subunit, wherein the large subunit has an amino acid sequence having no less than 90% identity to the amino acid sequence of the positions 26 to 390 of the amino acid sequence of SEQ ID NO:
- 2, wherein the glutamic acid at the position corresponding to position 38 of SEQ ID NO:
- 2 is modified by a substitution, wherein the small subunit has an amino acid sequence having no less than 90% sequence identity to the amino acid sequence of the positions 391 to 580 of the amino acid sequence of SEQ ID NO:
- 2, and wherein the tyrosine at the position corresponding to position 444 of SEQ ID NO:
- 2 is modified by a substitution.
Dependencies are inferred from the claim text.