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US 9,580,690 B2Granted
Attenuated recombinant alphaviruses incapable of replicating in mosquitoes and uses thereof
AssigneeBoard of Regents, The University of Texas System
InventorsScott C. Weaver, Ilya V. Frolov, Elena Frolova
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Abstract
The present invention discloses an attenuated recombinant alphavirus that is incapable of replicating in mosquito cells and of transmission by mosquito vectors. These attenuated alphavirus may include but is not limited to Western Equine Encephalitis virus, Eastern equine encephalitis virus, Venezuelan equine encephalitis virus or Chikungunya virus. The present invention also discloses the method of generating such alphaviruses and their use as immunogenic compositions.
Claims9
A live, attenuated Chikungunya (CHIK) virus comprising:
- a CHIK virus polynucleotide having (i) an inactivated subgenomic promoter, and (ii) an insertion of an encephalomyelocarditis virus internal ribosomal entry site (EMCV IRES) functional only in cells of vertebrate origin, between one end of nonstructural protein 4 (nsP4) coding sequence and initiating AUG of a subgenomic RNA coding sequence of the CHIK virus, wherein the subgenomic promoter is inactivated by clustered point mutations and wherein the CHIK virus is attenuated.
Dependencies are inferred from the claim text.