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US 9,572,836 B2Granted
Methods for assessing the suitability of transduced T cells for administration
AssigneeThe Trustees of the University of Pennsylvania
InventorsCarl H. June, Bruce L. Levine, Michael D. Kalos
Life of this patent
Estimating the term…
Abstract
The invention relates to of analyzing vector supernatants useful for transducing T cells destined for administration to a human subject. The invention also related to methods of analyzing transduced T cells destined for administration to a human subject.
Claims14
A method of analyzing a composition comprising a genetically modified T cell to detect a contaminant, the method comprises:
- preparing the composition comprising the genetically modified T cell, wherein the genetically modified T cell comprises a nucleic acid encoding a chimeric antigen receptor (CAR) comprising an antigen binding domain, a transmembrane domain, a costimulatory signaling region, and a signaling domain;
- and
- analyzing the composition for at least one contaminant selected from the group consisting of a replication competent lentivirus (RCL), p24, a nucleic acid and/or protein derived from a plasmid used to package a lentivirus plasmid, vesicular stomatitis virus G-protein (VSV-G) nucleic acid and/or protein, and human immunodeficiency virus (HIV) gag.
Dependencies are inferred from the claim text.