# US 9,567,652 B2 · Methods of murine astrovirus detection

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- Number: US 9,567,652 B2
- Document ID: US9567652B2
- Status: Granted
- Kind: B2
- Application number: 13/957,124
- Filed: 2013-08-01
- Published: 2014-06-26
- Granted: 2017-02-14
- Earliest claimed: 2012-08-01
- Assignees: Washington University
- Applicants: Washington University
- Inventors: Herbert W. Virgin; David Wang; Joy Loh; Thad Stappenbeck; Guoyan Zhao; Larissa Thackray; Christine Yokoyama
- Web page: https://patentgrep.com/patent/US9567652B2

## CPC

- C12Q 1/701: {Specific hybridization probes}
- C07K 14/005: from viruses
- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- C12N 2770/12021: Viruses as such, e.g. new isolates, mutants or their genomic sequences
- C12N 2770/12022: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C12N 2770/12023: Virus like particles [VLP]
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Novel murine astroviruses, and methods of detecting the viruses are disclosed. Also disclosed are uses of the viruses and infected animals as model systems for discovery and development of vaccines and therapies for diseases caused by or associated with astrovirus infection, including human astrovirus-based diseases.

## Claims

### Claim 1

A method of detecting the presence, absence or quantity of a murine astrovirus in a mouse sample, the method comprising:
providing a sample from a mouse comprising or suspected of comprising a murine astrovirus wherein the mouse sample is selected from the group consisting of a fecal sample, a vomitus sample, a tissue sample and a blood sample;
synthesizing cDNA using sample RNA as a template;
contacting the cDNA with at least one primer comprising a sequence having at least 95% sequence identity with
(SEQ ID NO: 6)
CCAAGAAAGAGGCACTAGTGGCACTC
(SEQ ID NO: 7)
GTTTTTTTTTTTTTTTTTTTTTGCCAATTTTTATGCCAATTATATCACCC
or
(SEQ ID NO: 9)
GTGTCACTAACGCGCACCTTTTCA;
and
performing a quantitative PCR assay comprising the cDNA and the at least one primer, thereby detecting the presence, absence or quantity of a murine astrovirus nucleic acid.

### Claim 2 (depends on 1)

A method of detecting the presence, absence or quantity of a murine astrovirus in a mouse sample in accordance with claim 1, wherein the at least one primer that has at least 95% sequence identity with SEQ ID NO:6, SEQ ID NO:7, or SEQ ID NO:9, has 100% sequence identity with SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:9.

### Claim 3 (depends on 1)

A method according to claim 1, wherein the sample is a fecal sample.