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US 9,567,645 B2Granted
Massively parallel single cell analysis
AssigneeCellular Research, Inc.
InventorsChristina Fan, Stephen P. A. Fodor, Glenn Fu, Geoffrey Richard Facer, Julie Wilhelmy
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Abstract
The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).
Claims28
A method comprising:
- associating a single bead with a single cell,
- wherein said single bead comprises a plurality of oligonucleotides,
- wherein each of said plurality of oligonucleotides comprises an identical cellular label sequence, a molecular label sequence, and a target-binding region, and
- wherein at least 100 of said plurality of oligonucleotides comprise different molecular label sequences.
1 more element
Dependencies are inferred from the claim text.