Non-caloric sweeteners and methods for synthesizing
AssigneeConagen Inc.
InventorsGuohong Mao, Xiaodan Yu
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Abstract
Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebaudioside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
Claims18
A synthetic rebaudioside consisting of a chemical structure:
A method for synthesizing rebaudioside V from rebaudioside G, the method comprising:
- preparing a reaction mixture comprising:
- (a) rebaudioside G,
- (b) substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), and
- (c) a HV1 UDP-glycosyltransferase having the amino acid sequence of SEQ ID NO:
- 5;
- and
- incubating the reaction mixture for a sufficient time to produce rebaudioside V, wherein a glucose is covalently coupled to the rebaudioside G to produce rebaudioside V.
A method for synthesizing rebaudioside V from rebaudioside G, the method comprising:
- preparing a reaction mixture comprising:
- (a) rebaudioside G,
- (b) substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), and
- (c) a uridine diphospho glycosyltransferase (UDP-glycosyltransferase) selected from the group consisting of an EUGT11 UDP-glycosyltransferase having the amino acid sequence of SEQ ID NO:
- 3 and a UDP-glycosyltransferase fusion enzyme having the amino acid sequence of SEQ ID NO:
- 11;
- and
- incubating the reaction mixture for a sufficient time to produce rebaudioside V, wherein a glucose is covalently coupled to the rebaudioside G to produce rebaudioside V.
A method for synthesizing rebaudioside V from rebaudioside KA, the method comprising:
- preparing a reaction mixture comprising:
- (a) rebaudioside KA,
- (b) substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), and
- (c) a uridine diphospho glycosyltransferase (UDP-glycosyltransferase) selected from the group consisting of a UGT76G1 UDP-glycosyltransferase having the amino acid sequence of SEQ ID NO:
- 1 and a UDP-glycosyltransferase fusion enzyme having the amino acid sequence of SEQ ID NO:
- 9;
- and
- incubating the reaction mixture for a sufficient time to produce rebaudioside V, wherein a glucose is covalently coupled to the rebaudioside KA to produce rebaudioside V.
A method for synthesizing rebaudioside V from rubusoside, the method comprising:
- preparing a reaction mixture comprising:
- (a) rubusoside,
- (b) substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), and
- (c) uridine diphospho glycosyltransferases (UDP-glycosyltransferases) selected from the group consisting of a UGT76G1 UDP-glycosyltransferase having the amino acid sequence of SEQ ID NO:
- 1, a HV1 UDP-glycosyltransferase having the amino acid sequence of SEQ ID NO:
- 5 and a UDP-glycosyltransferase fusion enzyme having the amino acid sequence of SEQ ID NO:
- 9;
- and incubating the reaction mixture for a sufficient time to produce rebaudioside V.
A method for synthesizing a rebaudioside V from rubusoside, the method comprising:
- preparing a reaction mixture comprising:
- (a) rubusoside,
- (b) substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), and
- (c) uridine diphospho glycosyltransferases (UDP-glycosyltransferases) selected from the group consisting of a UGT76G1 UDP-glycosyltransferase having the amino acid sequence of SEQ ID NO:
- 1, EUGT11 UDP-glycosyltransferase having the amino acid sequence of SEQ ID NO:
- 3 and a UDP-glycosyltransferase fusion enzyme having the amino acid sequence of SEQ ID NO:
- 11;
- and incubating the reaction mixture for a sufficient time to produce rebaudioside V.
A sweetener comprising a synthetic rebaudioside, the synthetic rebaudioside consisting of a chemical structure:
Dependencies are inferred from the claim text.