Method for producing carotenoids each having 50 carbon atoms
AssigneeNational University Corporation Chiba University
InventorsDaisuke Umeno, Maiko Furubayashi, Norihiko Misawa, Shinichi Takaichi
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Abstract
The present invention provides a method of producing a carotenoid having 50 carbon atoms which comprises culturing, in a medium, a cell transformed with a mutant phytoene desaturase gene and obtaining the carotenoid having 50 carbon atoms from the culture. The mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms.
Claims17
A method of producing a carotenoid having 50 carbon atoms, comprising:
- culturing in vitro, in a medium, a single cell organism transformed with a mutant phytoene desaturase gene;
- and
- obtaining the carotenoid having 50 carbon atoms from a culture after the culturing;
- wherein the mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms;
- and
- the mutation causes at least a substitution of an amino acid corresponding to asparagine at position 304 in SEQ ID NO:
- 1 by proline or serine, and the mutation consists of one or more substitutions at positions 228, 304, 338, 339, and 395 of SEQ ID NO:
- 1.
A method of producing a carotenoid having 50 carbon atoms, comprising:
- culturing in vitro, in a medium, a single cell organism transformed with a mutant phytoene desaturase gene;
- and
- obtaining the carotenoid having 50 carbon atoms from a culture after the culturing;
- wherein the mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms;
- and
- the mutation causes a substitution of an amino acid corresponding to at least one amino acid selected from asparagine at position 304, phenylalanine at position 339, isoleucine at position 338, aspartic acid at position 395, and isoleucine at position 228 in the amino acid sequence of SEQ ID NO:
- 1, and the mutation consists of one or more substitutions at positions 228, 304, 338, 339, and 395 of SEQ ID NO:
- 1.
A mutant phytoene desaturase gene, into which a mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms has been introduced, wherein the mutation of the mutant phytoene desaturase gene causes at least a substitution of an amino acid corresponding to asparagine at position 304 in SEQ ID NO:
- 1 by proline or serine, and the mutation consists of one or more substitutions at positions 228, 304, 338, 339, and 395 of SEQ ID NO:
- 1.
A mutant phytoene desaturase gene, into which a mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms has been introduced, wherein the mutation of the mutant phytoene desaturase gene causes a substitution of an amino acid corresponding to at least one amino acid selected from asparagine at position 304, phenylalanine at position 339, isoleucine at position 338, aspartic acid at position 395, and isoleucine at position 228 in the amino acid sequence of SEQ ID NO:
- 1, and the mutation consists of one or more substitutions at positions 228, 304, 338, 339, and 395 of SEQ ID NO:
- 1.
A method of producing a carotenoid having 50 carbon atoms, comprising:
- culturing in vitro, in a medium, a single cell organism transformed with a mutant phytoene desaturase gene;
- and
- obtaining the carotenoid having 50 carbon atoms from a culture after the culturing;
- wherein the mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms;
- and
- the mutation causes a substitution of an amino acid corresponding to phenylalanine at position 339 in SEQ ID NO:
- 1 by serine, a substitution of an amino acid corresponding to asparagine at position 304 by serine, substitutions of an amino acid corresponding to phenylalanine at position 339 by lysine and an amino acid corresponding to aspartic acid at position 395 with asparagine, substitutions of an amino acid corresponding to isoleucine at position 228 by valine and an amino acid corresponding to isoleucine at position 338 by valine, or a substitution of an amino acid corresponding to isoleucine at position 338 by valine, and the mutation consists of one or more substitutions at positions 228, 304, 338, 339, and 395 of SEQ ID NO:
- 1.
A mutant phytoene desaturase gene, into which a mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms has been introduced, wherein the mutation of the mutant phytoene desaturase gene causes a substitution of an amino acid corresponding to phenylalanine at position 339 in SEQ ID NO:
- 1 by serine, a substitution of an amino acid corresponding to asparagine at position 304 by serine, substitutions of an amino acid corresponding to phenylalanine at position 339 by lysine and an amino acid corresponding to aspartic acid at position 395 with asparagine, substitutions of an amino acid corresponding to isoleucine at position 228 by valine and an amino acid corresponding to isoleucine at position 338 by valine, or a substitution of an amino acid corresponding to isoleucine at position 338 by valine, and the mutation consists of one or more substitutions at positions 228, 304, 338, 339, and 395 of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.