Methods of screening for binding interaction using sets of microparticles and unique probes
AssigneeOne Lambda
InventorsJar-How Lee, Terrence Chen, Thoa Nong
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Abstract
The present invention relates to a kit for conducting a method of measuring binding interactions using multiple sets of microparticles, wherein said set has the same identifiable characteristic and wherein one of more sets comprise subsets of microparticles and said subset presents at least one unique probe that acts as a binding partner for a target molecule in a biological sample. In particular, the invention provides for a kit for conducting a method of DNA-based tissue-typing antigens in donor tissue or recipient tissue using these multiple sets of microparticles.
Claims11
A kit for conducting a method of measuring a binding interaction comprising:
- multiple sets of microparticles, the microparticles of each set having an identifiable characteristic unique to each set such that each set is distinguishable from the other sets, wherein each set comprises at least two subsets of microparticles presenting a probe unique to each subset, wherein the probe of each subset binds to a different target molecule within a biological sample from the target molecule bound by other subsets, forming a binding complex with the target molecule;
- and
- a labeling reagent for labeling the binding complex with a detectable label that produces a measurable signal when a specific binding complex is present, and wherein the signal varies in intensity such that only the microparticles exhibiting greatest signal intensity, which comprises a proportion of less than 100% of the microparticles, are selected for analysis.
A kit for identifying tissue typing antigen alleles from a biological sample, the kit comprising:
- multiple sets of microparticles, the microparticles of each set having an identifiable characteristic unique to each set such that each set is distinguishable from the other sets, wherein each set comprises at least two subsets of microparticles presenting a sequence specific oligonucleotide (SSO) or primer unique to each subset, wherein the SSO or primer of each subset comprises a sequence selected to hybridize with a different tissue-typing antigen allele which may be present in the sample to form one or more hybridization products, and
- a labeling reagent for labeling the one or more hybridization products with a detectable label that produces a measurable signal when a specific hybridization product is present, and wherein the signal varies in intensity such that only the microparticles exhibiting greatest signal intensity, which comprises a proportion of less than 100% of the microparticles, are selected for analysis.
A kit for identifying human leukocyte antigen (HLA) alleles from a biological sample, the kit comprising:
- multiple sets of microparticles, the microparticles of each set having an identifiable characteristic unique to each set, wherein each set comprises at least two subsets of microparticles presenting a sequence specific oligonucleotide (SSO) unique to each subset, wherein the SSO of each subset comprises a sequence selected to hybridize with a different HLA allele which may be present in the sample to form one or more hybridization products, and
- a labeling reagent for labeling the one or more hybridization products with a detectable label that produces a measurable signal when a specific hybridization product is present, and wherein the signal varies in intensity such that only the microparticles exhibiting greatest signal intensity, which comprises a proportion of less than 100% of the microparticles, are selected for analysis.
Dependencies are inferred from the claim text.