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US 9,556,470 B2Granted
Enzyme quantification
AssigneeRaindance Technologies, Inc.
InventorsDarren Link, Michael L. Samuels
Life of this patent
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Abstract
The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.
Claims25
A method for quantifying enzyme molecules, the method comprising:
- forming a plurality of fluid partitions comprising enzyme molecules and substrates in a microfluidic channel, wherein at least one substrate comprises a detectable label;
- conducting an enzymatic reaction in at least one of the fluid partitions, wherein the detectable label is released;
- and
- quantifying the enzyme molecules by determining a number of fluid partitions that released the detectable label.
1 more element
Dependencies are inferred from the claim text.