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US 9,556,460 B2Granted
Method for the production of 3-hydroxy-3-methylbutyric acid from acetone and an activated acetyl compound
AssigneeScientist of Fortune, S.A.
InventorPhilippe Marliere
Life of this patent
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Abstract
Described is a method for the production of 3-hydroxy-3-methylbutyric acid by enzyme-catalyzed covalent bond formation between the carbon atom of the oxo group of acetone and the methyl group of a compound which provides an activated acetyl group. Also described are recombinant organisms which produce 3-hydroxy-3-methylbutyric acid, and related compositions and methods.
Claims21
A method of producing 3 hydroxy-3-methylbutyric acid comprising:
- (a) providing a recombinant microorganism, wherein the recombinant microorganism is genetically modified to overexpress a polynucleotide encoding a 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase (EC 2.3.3.10) as compared to the same recombinant microorganism without the genetic modification, and wherein the recombinant microorganism is genetically modified to overexpress a polynucleotide encoding an acetoacetate decarboxylase as compared to the same recombinant microorganism without the genetic modification;
- and
- (b) incubating, in the recombinant microorganism (i) acetone, (ii) acetyl-coenzyme A, and (iii) the HMG-CoA synthase, to thereby produce 3-hydroxy-3-methylbutyric acid.
A recombinant microorganism which:
- (a) produces acetone and acetyl-coenzyme A;
- (b) is genetically modified to overexpress a polynucleotide encoding a 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase (EC 2.3.3.10) as compared to the same recombinant microorganism without the genetic modification;
- (c) is genetically modified to overexpress a polynucleotide encoding an acetoacetate decarboxylase as compared to the same recombinant microorganism without the genetic modification;
- and
- (d) produces 3-hydroxy-3-methylbutyric acid from acetone and acetyl-coenzyme A.
2 more elements
Dependencies are inferred from the claim text.