Specialized (iso)eugenol-4-O-methyltransferases (s-IEMTs) and methods of making and using the same
AssigneeBrookhaven Science Associates, LLC
InventorsChang-Jun Liu, Yuanheng Cai
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Abstract
Specialized (iso)eugenol 4-O-methyltransferase (s-IEMT) enzymes having increased capacity for methylation of monolignols are disclosed. The s-IEMTs have unique activity favoring methylation of coniferyl alcohol versus sinapyl alcohol. Various s-IEMTs methylate ferulic acid. Means for producing the various s-IEMTs are provided. The s-IEMTs are useful for modification of lignin content and production of aromatic compounds.
Claims12
A specialized (iso)eugenol 4-O-methyltransferase (s-IEMT) enzyme comprising amino acid substitutions at each of residues 133, 165, 175, 166, 169, 26, 30 and 33, where the amino acid residues correspond to those of SEQ ID NO. 1.
An isolated nucleic acid sequence comprising a sequence encoding a specialized (iso)eugenol 4-O-methyltransferase enzyme having amino acid substitutions at each of residues 133, 165, 175, 166, 169, 26, 30 and 33 of SEQ ID NO. 1.
Dependencies are inferred from the claim text.