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US 9,550,999 B2Granted
Recombinant host cells comprising phosphoketolases
AssigneeButamax Advanced Biofuels LLC
InventorsMichael Dauner, Lori Ann Maggio-Hall, Jean-Francois Tomb
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Abstract
The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate, or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.
Claims22
A recombinant host cell comprising:
- (i) at least one genetic modification in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity, wherein the at least one genetic modification reduces or eliminates pyruvate decarboxylase activity;
- and
- (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity.
Dependencies are inferred from the claim text.