Humanized anti-HMGB1 antibody or antigen-binding fragment thereof

A humanized antibody specifically binding to an amino acid sequence (EEEDDDDE (SEQ ID NO:

• 60)) present in the C-terminal domain of HMGB1 protein, or an antigen-binding fragment thereof, which capable of neutralizing the biological activity of HMGB1 protein, comprising a heavy chain variable region (VH) and a light chain variable region (VL), wherein
• (i) the heavy chain variable region (VH) comprises:
• (a) an amino acid sequence of heavy chain CDR1 comprising the amino acid sequence of SEQ ID NO:
• 7;
• (b) an amino acid sequence of heavy chain CDR2 comprising the amino acid sequence of SEQ ID NO:
• 8;
• (c) an amino acid sequence of heavy chain CDR3 comprising the amino acid sequence of SEQ ID NO:
• 9;
• (d) amino acid sequences of SEQ ID Nos:
• 43, 44, 45, and 46 as amino acid sequences of FR1, FR2, FR3, and FR4, respectively, wherein the amino acid sequences of FR1, FR2, FR3, and FR4 optionally have mutations of deletion, substitution and/or addition of one or several amino acid residues in the amino acid sequences of SEQ ID NOs:
• 43, 44, 45 and 46, respectively;
• and
• (ii) the light chain variable region (VL) comprises:
• (a) an amino acid sequence of light chain CDR1 comprising the amino acid sequence of SEQ ID NO:
• 10,
• (b) an amino acid sequence of light chain CDR2 comprising the amino acid sequence of SEQ ID NO:
• 11;
• (c) an amino acid sequence of light chain CDR3 comprising the amino acid sequence of SEQ ID NO:
• 12;
• and
• (d) the light chain variable region (VL) further comprises the amino acid sequences of SEQ ID Nos:
• 47, 48, 49 and 50 as amino acid sequences of FR1, FR2, FR3, and FR4, respectively, wherein the amino acids sequences of FR1, FR2, FR3, and FR4 optionally have mutations of deletion, substitution, insertion, and/or addition of one to several amino acid residues in the amino acid sequences of SEQ ID NOs:
• 47, 48, 49, and 50, respectively.