Multicistronic expression constructs
AssigneeUniversity of Massachusetts
InventorsGuangping Gao, Jun Xie
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Abstract
Some aspects of this invention provide nucleic acid constructs for transgene expression. Some aspects of this invention provide multicistronic nucleic acid constructs, for example, comprising an expression cassette encoding a hairpin RNA and a reporter expression cassette. Some aspects of this invention provide nucleic acid constructs comprising two or more self-complementary nucleic acid sequences, for example, hairpin RNA encoding nucleic acid sequences and AAV inverse terminal repeats. Methods for the use of the constructs in therapy and research are also provided.
Claims23
A recombinant nucleic acid construct, comprising
- a recombinant AAV (rAAV) vector comprising inverted terminal repeats (ITRs);
- a first expression cassette, comprising a nucleic acid encoding a gene product heterologous to the vector under the control of a first promoter positioned between the ITRs of the vector, and an intron, wherein the intron is positioned between the transcriptional start site of the first expression cassette and the nucleic acid encoding the gene product, and
- a second expression cassette, comprising a self-complementary nucleic acid sequence under the control of a second promoter, wherein the second expression cassette is positioned within the intron of the first expression cassette in proximity with the first promoter.
Dependencies are inferred from the claim text.