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US 9,545,440 B2Granted
Methods for preparing squalene
AssigneeNovartis AG
InventorManinder Hora
Life of this patent
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Abstract
An improved method for preparing squalene from a squalene-containing composition, said method comprising the steps of (a) a purification distillation carried out at a temperature T1 (b) a denaturing distillation carried out at a temperature T2; wherein steps (a) and (b) may be performed in either order; T1 and T2 are sufficient to cause squalene to boil; T2>T1; and T2>200° C.
Claims47
A method for the manufacture of an oil-in-water emulsion comprising:
- i. obtaining squalene prepared from a fish oil source by a process comprising subjecting fish oil comprising squalene to:
- (a) a purification distillation carried out at near vacuum;
- and (b) a denaturing distillation carried out at a temperature greater than or equal to 210° C. at a pressure of 0.8 to 5.0 mmHg,
- wherein the temperature in the denaturing distillation exceeds the temperature in the purification distillation;
- and
- wherein the conditions for both the purification and the denaturing distillation are sufficient to cause squalene to boil;
- and
- wherein the denaturing distillation denatures and/or removes potential contaminant proteins and viruses, thereby rendering the squalene safe for human use as compared with non-denatured squalene;
- and
- ii. preparing an oil-in-water emulsion using the squalene from step (i).
7 more elements
A method for processing squalene from a fish source, said method comprising the steps of:
- i providing a composition comprising distilled squalene which has been obtained by a process of distillation, and wherein the squalene is from a fish source;
- and
- ii re-distilling the composition from (i) at a temperature sufficient to cause the distilled squalene to boil, so as to provide a composition comprising re-distilled squalene;
- wherein the process of distillation is performed at a temperature less than 140° C. and at a near vacuum and the process of re-distillation is performed at a temperature greater than or equal to 210° C. and a pressure of 0.8 to 5.0 mmHg;
- and
- wherein the conditions for both the distillation and the re-distillation are sufficient to cause the squalene to boil;
- and
- wherein the denaturing distillation denatures and/or removes potential contaminant proteins and viruses, thereby rendering the squalene safer for human use as compared with non-denatured squalene.
5 more elements
A method for the manufacture of an oil-in-water emulsion comprising:
- i. obtaining squalene prepared from a fish oil source by a process comprising subjecting fish oil comprising squalene to:
- (a) a purification distillation carried out at near vacuum at a temperature of less than 140° C.;
- and (b) a denaturing distillation carried out at a temperature greater than or equal to 210° C.,
- wherein the conditions for both the purification and the denaturing distillation are sufficient to cause squalene to boil;
- and
- wherein the denaturing distillation denatures and/or removes potential contaminant proteins and viruses, thereby rendering the squalene safe for human use as compared with non-denatured squalene;
- and
- ii. preparing an oil-in-water emulsion using the squalene from step (i).
5 more elements
A method for the manufacture of an oil-in-water emulsion comprising:
- i. obtaining squalene prepared from a fish oil source by a process comprising subjecting fish oil comprising squalene to:
- (a) a purification distillation carried out at near vacuum;
- and (b) a denaturing distillation carried out at a temperature greater than or equal to 210° C. at a pressure of 0.8 to 5.0 mmHg,
- wherein the temperature in the denaturing distillation exceeds the temperature in the purification distillation;
- and
- wherein the conditions for both the purification and the denaturing distillation are sufficient to cause squalene to boil;
- and
- wherein the denaturing distillation denatures and/or removes potential contaminant proteins and viruses, thereby rendering the squalene safe for human use as compared with non-denatured squalene;
- and
- ii. preparing an oil-in-water emulsion using the squalene from step (i) by:
- (a) preparing a first emulsion having a first average droplet size;
- (b) microfluidizing the first emulsion to form a second emulsion having a second average droplet size which is less than the first average oil droplet size;
- and (c) filtering the second emulsion;
- wherein the oil droplets in the oil-in-water emulsion have a diameter less than 5 μm.
11 more elements
Dependencies are inferred from the claim text.