US 9,545,438 B2 · Determination of antigenicity of an inactivated virus

Abstract

The invention relates generally to the field of virology. More particularly, the present invention relates to methods for determining the effect of a viral inactivation procedure on the antigenicity of the inactivated virus, in particular, for a virus that is a member of the family Arteriviridae or Coronaviridae or Asfarviridae, in particular, for Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). The invention further provides methods to determine the antigenicity of an inactivated virus as well as methods to screen for anti-viral compounds using any one of the aforementioned methods. Methods of using the inactivated and immunogenic virus thus obtained, in particular, in the manufacture of a vaccine, are also provided by the present invention.

Claims16

Dependent claims

A method of determining an inactivated virus's antigenicity, wherein the inactivated virus is Porcine Reproductive and Respiratory Syndrome (PRRS) virus, the method comprising:

purposefully inactivating a PRRS virus with a procedure able to inactivate the virus without affecting the thus inactivated virus's ability to bind and/or to enter and/or to fuse with the host cell;
contacting a host cell with the inactivated PRRS virus in vitro;
detecting the binding to and/or the internalization into the host cell and/or the fusion of the inactivated virus with the host cell;
and
determining that the inactivated virus as having has antigenicity if the inactivated virus is able to bind to and/or enter and/or fuse with the host cell.

2 more elements

Dependencies are inferred from the claim text.

Determination of antigenicity of an inactivated virus

Life of this patent

Determination of antigenicity of an inactivated virus

Life of this patent

Abstract

Claims16