Bioactive compositions and methods for their preparation and use
AssigneeBioregenerative Sciences
InventorGreg Maguire
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Abstract
A method for preparing a bioactive composition containing conditioned cell culture medium is disclosed. The method comprises culturing cells of two or more eukaryotic cell line to form conditioned culture media, separating the cultured cells from the conditioned culture media, and combining conditioned culture media to form a bioactive composition. Novel bioactive compositions, formulations and their use in treating of a variety of diseases and health conditions are also disclosed.
Claims21
A method for treating an eye condition in a subject in need thereof, comprising administering to said subject a therapeutically effective amount of a bioactive formulation that comprises a composition prepared by
- culturing cells of a first cell line and a second cell line in a first and a second culture medium, wherein said cultured cells secrete extracellular products into said culture medium so that a first conditioned culture medium and a second conditioned culture medium are formed;
- separating said first and second conditioned culture media from respective said cultured cells;
- and
- combining said first and said second conditioned culture media to form the composition,
- wherein said first and second cell lines are an adult human adipose derived stem cell (ADSC) line and a fetal human fibroblast cell line.
Dependencies are inferred from the claim text.