Multi-dimensional survey scans for improved data dependent acquisitions
AssigneeMicromass UK Limited
InventorsKevin Giles, Jason Lee Wildgoose
Life of this patent
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Abstract
A method of analyzing ions is disclosed comprising performing an initial multi-dimensional survey scan comprising separating parent ions according to a first physico-chemical property (e.g. ion mobility) and then separating the parent ions according to a second physico-chemical property (e.g. mass to charge ratio). A plurality of parent ions of interest are then determined from the initial multi-dimensional survey scan. Once parent ions of interest have been determined, the plurality of parent ions of interest are sequentially selected based upon the first and second physico-chemical properties during a single cycle of separation. The parent ions of interest may then be fragmented and corresponding fragment ions may then be mass analyzed.
Claims28
A method of analysing ions in a Data Dependent Acquisition (DDA) experiment comprising:
- performing an initial multi-dimensional survey scan comprising separating parent ions according to a first physico-chemical property and separating said parent ions according to a second physico-chemical property;
- determining a plurality of parent ions of interest to be selected for the DDA experiment from said initial multi-dimensional survey scan;
- and then
- sequentially selecting and transmitting said plurality of parent ions of interest based upon said first physico-chemical property and said second physico-chemical property during a single acquisition or cycle of separation;
- and
- analysing either:
- (i) one or more of said plurality of parent ions of interest;
- or (ii) one or more fragment, product or other ions derived from one or more of said plurality of parent ions of interest.
An analytical instrument for analysing ions in a Data Dependent Acquisition (DDA) experiment comprising:
- a first separator or filter for separating or filtering ions according to a first physico-chemical property;
- a second separator or filter for separating or filtering ions according to a second physico-chemical property;
- an analyser;
- and
- a control system arranged and adapted:
- (i) to perform an initial multi-dimensional survey scan comprising separating parent ions according to said first physico-chemical property using said first separator and separating said parent ions according to said second physico-chemical property using said second separator;
- (ii) to determine a plurality of parent ions of interest to be selected for the DDA experiment from said initial multi-dimensional survey scan;
- and then
- (iii) to sequentially select and transmit said plurality of parent ions of interest based upon said first physico-chemical property and said second physico-chemical property during a single acquisition or cycle of separation;
- and
- (iv) to cause said analyser to analyse either:
- (i) one or more of said plurality of parent ions of interest;
- or (ii) one or more fragment, product or other ions derived from one or more of said plurality of parent ions of interest.
A method of analysing ions in a Data Dependent Acquisition (DDA) experiment comprising:
- performing an initial multi-dimensional survey scan comprising separating parent ions according to a first physico-chemical property and separating said parent ions according to a second physico-chemical property;
- determining a plurality of parent ions of interest to be selected for the DDA experiment from said initial multi-dimensional survey scan;
- and then fragmenting said parent ions to generate a plurality of first generation fragment ions;
- sequentially selecting and transmitting a plurality of first generation fragment ions of interest based upon said first physico-chemical property and said second physico-chemical property during a single acquisition or cycle of separation;
- fragmenting said plurality of first generation fragment ions of interest to generate a plurality of second generation fragment ions;
- and
- analysing either:
- (i) said second generation fragment ions;
- or (ii) fragment, product or other ions derived from said second generation fragment ions.
A mass spectrometer for use in a Data Dependent Acquisition (DDA) experiment comprising:
- a first separator or filter for separating or filtering ions according to a first physico-chemical property;
- a second separator or filter for separating or filtering ions according to a second physico-chemical property;
- a fragmentation device;
- and
- a control system arranged and adapted:
- (i) to perform an initial multi-dimensional survey scan comprising separating parent ions according to said first physico-chemical property and separating said parent ions according to said second physico-chemical property;
- (ii) to determine a plurality of parent ions of interest to be selected for the DDA experiment from said initial multi-dimensional survey scan;
- and then
- (iii) to cause said parent ions to fragment within said fragmentation device to generate a plurality of first generation fragment ions;
- (iv) to sequentially select and transmit a plurality of first generation fragment ions of interest based upon said first physico-chemical property and said second physico-chemical property during a single acquisition or cycle of separation;
- (v) to cause said plurality of first generation fragment ions of interest to fragment to generate a plurality of second generation fragment ions;
- and
- (vi) to analyse either:
- (i) said second generation fragment ions;
- or (ii) fragment, product or other ions derived from said second generation fragment ions.
Dependencies are inferred from the claim text.