Reagent for assaying D-dimer and kit of reagent for assaying D-dimer
AssigneeSysmex Corporation
InventorsKeisuke Nagai, Kazuaki Yamashita, Susumu Hoshiko, Takeshi Suzuki
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Abstract
The present invention provides a reagent for assaying D-dimer which includes carriers sensitized to first and second monoclonal antibodies which react with D-dimer, but have different reactivity to D-dimer in which the first monoclonal antibody reacts with high- and low-molecular fractions of D-dimer, the second monoclonal antibody reacts with the high-molecular fraction, but reactivity of the second monoclonal antibody with the low-molecular fraction is different from that of the first monoclonal antibody and a kit of reagent for assaying D-dimer.
Claims20
A method of assaying D-dimer in a biological sample comprising:
- mixing the biological sample and a reagent comprising carrier particles comprising a first particle sensitized to a first monoclonal antibody which reacts with D-dimer and a second particle sensitized to a second monoclonal antibody which reacts with D-dimer;
- and
- measuring a level of aggregation of the particles caused by an antigen-antibody reaction between D-dimer and the first and second monoclonal antibodies in the mixture, wherein
- the first monoclonal antibody reacts with X-fraction, Y-fraction, and D-dimer, but does not react with D- and E-fractions, and
- the second monoclonal antibody reacts with D-dimer, but does not react with X-, Y-, and E-fractions.
A method of assaying D-dimer in a biological sample comprising:
- forming a mixture by mixing the biological sample and a reagent comprising carrier particles sensitized to first and second monoclonal antibodies which react with D-dimer, but have different reactivity to D-dimer;
- and
- measuring a level of aggregation of the particles caused by an antigen-antibody reaction between D-dimer and the first and second monoclonal antibodies in the mixture, wherein
- the first monoclonal antibody reacts with X-fraction, Y-fraction, and D-dimer, but does not react with D- and E-fractions, and
- the second monoclonal antibody reacts with D-dimer, but does not react with X-, Y-, and E-fractions.
A method of assaying D-dimer in a biological sample comprising:
- forming a mixture by mixing the biological sample and a reagent comprising carrier particles comprising a first particle sensitized to a first monoclonal antibody which reacts with D-dimer and a second particle sensitized to a second monoclonal antibody which reacts with D-dimer;
- measuring a level of aggregation of the particles caused by an antigen-antibody reaction between D-dimer and the first and second monoclonal antibodies in the mixture;
- and
- calculating concentration and/or amount of D-dimer in the biological sample based on the level of aggregation and a calibration curve obtained by measuring a concentration-known D-dimer reference material, wherein
- the first monoclonal antibody reacts with X-fraction, Y-fraction, and D-dimer, but does not react with D- and E-fractions, and
- the second monoclonal antibody reacts with D-dimer, but does not react with X-, Y-, and E-fractions.
Dependencies are inferred from the claim text.