Mass spectrometry assay for congenital adrenal hyperplasia
AssigneeQuest Diagnostics Investments Incorporated
InventorsAmit Ghoshal, Nigel J. Clarke, Mildred M. Goldman
Life of this patent
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Abstract
Methods are provided for detecting the amount of one or more CAH panel analytes (i.e., pregnenolone, 17-OH pregnenolone, progesterone, 17-OH progesterone, dehydroepiandrosterone (DHEA), androstenedione, testosterone, deoxycorticosterone, 11-deoxycortisol, and cortisol) in a sample by mass spectrometry. The methods generally involve ionizing one or more CAH panel analytes in a sample and quantifying the generated ions to determine the amount of one or more CAH panel analytes in the sample. In methods where amounts of multiple CAH panel analytes are detected, the amounts of multiple analytes are detected in the same sample injection.
Claims36
A method for determining the amount of a panel of analytes in a sample by mass spectrometry, the method comprising:
- ionizing the sample under conditions suitable to produce one or more ions detectable by mass spectrometry from each of the analytes;
- determining by tandem mass spectrometry the amount of the one or more ions from each of the analytes;
- and
- using the determined amount of the one or more ions to determine the amount of each of the analytes in the sample;
- wherein the analytes comprise 17-OH progesterone and androstenedione.
A method for determining the amount of a panel of analytes in a sample by mass spectrometry, the method comprising:
- ionizing the sample under conditions suitable to produce one or more ions detectable by mass spectrometry from each of the analytes;
- determining by tandem mass spectrometry the amount of the one or more ions from each of the analytes;
- and
- using the determined amount of the one or more ions to determine the amount of each of the analytes in the sample;
- wherein the analytes comprise at least two analytes selected from pregnenolone, 17-OH pregnenolone, progesterone, 17-OH progesterone, dehydroepiandrosterone (DHEA), androstenedione, testosterone, 11-deoxycortisol, deoxycorticosterone, corticosterone, cortisone, and cortisol.
Dependencies are inferred from the claim text.