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US 9,541,554 B2Granted
Detection of HIV-related proteins in urine
AssigneeMorehouse School of Medicine
InventorsGale W. Newman, Mike Powell, Akins Doherty, Chamberlain Obialo
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Abstract
A method for detecting HIV infection in a mammal is disclosed. The method contains the steps of isolating exosomes from a urine sample of a mammal and detecting the presence of HIV-specific biomarker in said isolated exosomes. A method for diagnosing a mammal with an HIV-associated disease, in particular, HIV-associated nephropathy is also disclosed.
Claims6
A method for detecting an HIV antigen in a human urine sample, comprising:
- isolating exosomes from a urine sample of a human subject by filtration of said urine sample through a filter having a pore size that is smaller than the exosomes;
- dissolving said exosomes in a liquid medium comprising a denaturing agent;
- testing for the presence of an HIV antigen selected from the group consisting of Nef, Gag and Pol in said exosomes by one or more techniques selected from the group consisting of electrophoresis, ELISA, Western blot, HPLC, FPLC, mass spectrometry (MS) and protein sequencing, and
- determining whether said HIV antigen is present in said exosomes of said urine sample based on said testing.
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Dependencies are inferred from the claim text.