US 9,541,551 B2 · Anti-GAP43 antibody

Abstract

An anti-GAP43 antibody which is capable of distinguishing a non-phosphorylated threonine residue at position 89 (T89) from a phosphorylated threonine residue at position 89 (pT89) of mouse GAP43 set forth in SEQ ID NO: 13, and which is capable of specifically detecting a growth cone; an anti-GAP43 antibody which is capable of distinguishing a non-phosphorylated serine residue at position 96 (S96) from a phosphorylated serine residue at position 96 (pS96) of mouse GAP43, and which is capable of specifically detecting a growth cone; an anti-GAP43 antibody which is capable of distinguishing a non-phosphorylated threonine residue at position 172 (T172) from a phosphorylated threonine residue at position 172 (pT172) of mouse GAP43, and which is capable of specifically detecting a growth cone; and an immunological analysis method using these anti-GAP43 antibodies.

Claims15

Dependent claims

A method of producing an anti-GAP43 antibody which is capable of distinguishing a non-phosphorylated threonine residue at position 89 (T89) from a phosphorylated threonine residue at position 89 (pT89) of mouse GAP43 set forth in SEQ ID NO:

13, and which is capable of specifically detecting a growth cone, the method comprising:
injecting a polypeptide comprising at least a part of the mouse GAP43 set forth in SEQ ID NO:
13 to an animal;
collecting antibodies from the animal;
subjecting the antibodies to affinity purification;
and
studying the purified antibodies for a cross reactivity thereof and selecting an antibody which shows cross reactivity against a mouse GAP43 having a phosphorylated threonine residue at position 89 (pT89) and which does not show cross reactivity against a non-phosphorylated mouse GAP43 or a mouse GAP43 having a phosphorylated residue other than the threonine residue at position 89.

4 more elements

A method of producing an anti-GAP43 antibody which is capable of distinguishing a non-phosphorylated serine residue at position 96 (S96) from a phosphorylated serine residue at position 96 (pS96) of mouse GAP43 set forth in SEQ ID NO:

13, and which is capable of specifically detecting a growth cone, the method comprising:
injecting a polypeptide comprising at least a part of the mouse GAP43 set forth in SEQ ID NO:
13 to an animal;
collecting antibodies from the animal;
subjecting the antibodies to affinity purification;
and
studying the purified antibodies for a cross reactivity thereof and selecting an antibody which shows cross reactivity against a mouse GAP43 having a phosphorylated serine residue at position 96 (pS96) and which does not show cross reactivity against a non-phosphorylated mouse GAP43 or a mouse GAP43 having a phosphorylated residue other than the serine residue at position 96.

4 more elements

A method of producing an anti-GAP43 antibody which is capable of distinguishing a non-phosphorylated threonine residue at position 172 (T172) from a phosphorylated threonine residue at position 172 (pT172) of mouse GAP43 set forth in SEQ ID NO:

13, and which is capable of specifically detecting a growth cone, the method comprising:
injecting a polypeptide comprising at least a part of the mouse GAP43 set forth in SEQ ID NO:
13 to an animal;
collecting antibodies from the animal;
subjecting the antibodies to affinity purification;
and
studying the purified antibodies for a cross reactivity thereof and selecting an antibody which shows cross reactivity against a mouse GAP43 having a phosphorylated threonine residue at position 172 (pT172) and which does not show cross reactivity against a non-phosphorylated mouse GAP43 or a mouse GAP43 having a phosphorylated residue other than the threonine residue at position 172.

4 more elements

Dependencies are inferred from the claim text.

Anti-GAP43 antibody

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Anti-GAP43 antibody

Life of this patent

Abstract

Claims15