Determination of a midregional proadrenomedullin partial peptide in biological fluids for diagnostic purposes, and immunoassays for carrying out such a determination
AssigneeB.R.A.H.M.S GmbH
InventorsAndreas Bergmann, Joachim Struck
Life of this patent
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Abstract
Method for the determination of adrenomedullin immunoreactivity in biological fluids for diagnostic purposes, in particular in sepsis, cardiac and cancer diagnosis, in which the midregional partial peptide (mid-proAM; SEQ ID NO:3) of proadrenomedullin, which comprises the amino acids (45-92) of the complete preproadrenomedullin (pre-proAM; SEQ ID NO:1), is measured in particular by means of an immunoassay which operates with at least one labelled antibody which specifically recognizes a sequence of mid-proAM.
Claims5
A method for the determination of the mid-regional partial peptide of proadrenomedullin (mid-proAM) in a patient employing at least two antibodies capable of binding within the region of amino acids 60-92 of preproadrenomedullin (SEQ ID NO:1),
- wherein the method comprises conducting a two-sided immunoassay by contacting plasma of the patient with two antibodies that specifically bind to different partial sequences of mid-proAM (SEQ ID NO:
- 3), so as to form sandwich complexes comprising the two antibodies and mid-proAM;
- and
- detecting the sandwich complexes, thereby determining mid-proAM;
- wherein one of the antibodies is obtained by immunization of an animal with an antigen which contains a synthetic peptide sequence which consists of SEQ ID NO:
- 4, and the other of the antibodies is obtained by immunization with an antigen which contains a synthetic peptide sequence which consists of SEQ ID NO:
- 5.
Dependencies are inferred from the claim text.