Enhancing visual assessment of samples
AssigneeCambridge Research & Instrumentation, Inc.
InventorClifford C. Hoyt
Life of this patent
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Abstract
Methods and systems for imaging a biological sample are disclosed, and include: (a) staining the sample with a first stain that includes eosin and at least one additional stain; (b) directing excitation light to the sample to cause each of the stains to emit fluorescence; and (c) recording images of the fluorescence emitted from the stains in the sample, where the amount of eosin in the sample is sufficiently dilute to cause the sample to have an average optical density of less than 0.10 at green wavelengths.
Claims34
A method for imaging a biological sample, the method comprising:
- a. staining the sample with a first stain comprising eosin and at least one additional stain;
- b. directing excitation light to the sample to cause each of the stains to emit fluorescence;
- and
- c. recording images of the fluorescence emitted from the stains in the sample,
- wherein the amount of eosin in the sample is sufficiently dilute to cause the sample to have an average optical density of less than 0.10 at green wavelengths.
A method for imaging a biological sample, the method comprising:
- a. staining the sample with a first stain comprising eosin, a nuclear counterstain, and at least one immunofluorescent stain, wherein the amount of eosin in the sample is sufficiently dilute to cause the sample to have an average optical density of less than 0.10 at green wavelengths;
- b. directing excitation light to the sample to cause each of the stains to emit fluorescence, wherein the amount of eosin in the sample is sufficient to produce detectable fluorescence by the eosin in response to the excitation light;
- c. recording images of the fluorescence emitted from the stains in the sample;
- d. computationally processing the recorded images to produce a synthetic image;
- and
- e. displaying the synthetic image.
A method for imaging a biological sample, the method comprising:
- a. staining the sample with a first stain comprising eosin and at least one additional stain;
- b. measuring fluorescence emission from eosin and from the at least one additional stain in the sample;
- and
- c. spectrally unmixing the measured fluorescence emission into contributions from eosin and from the at least one additional stain,
- wherein the amount of eosin in the sample is sufficiently dilute to cause the sample to have an average optical density of less than 0.10 at green wavelengths.
Dependencies are inferred from the claim text.