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US 9,541,481 B2Granted
Apparatus, system, and method for collecting a target material
AssigneeRareCyte, Inc.
InventorsDaniel Campton, Joshua Nordberg, Steve Quarre, David Stewart, Ronald Seubert, Jonathan Lundt, Lance U'Ren, Jennifer Chow
Life of this patent
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Abstract
This disclosure is directed to an apparatus, system and method for retrieving a target material from a sample. A first depletion agent may be added to a vessel that contains the sample to change the density of a non-target material.
Claims19
A method comprising the steps of:
- inserting a device comprising a cannula into an open end of a primary vessel comprising a suspension comprising a target material, wherein the device extends upwardly from the open end of the primary vessel;
- providing a processing vessel comprising
- a first end comprising a resealable plug, and
- an inner cavity;
- mating the processing vessel with the device, wherein the cannula extends through the resealable plug of the processing vessel and accesses the inner cavity of processing vessel and wherein the processing vessel extends upwardly from the device;
- adding a displacement fluid comprising a density greater than a density of the target material to the inner cavity of the processing vessel;
- and
- centrifuging the primary vessel, the device, the displacement fluid, and the processing vessel, wherein during centrifuging, the displacement fluid flows into the primary vessel via the device and displaces the target material from the primary vessel, which flows into the processing vessel through the device via the cannula.
5 more elements
A system comprising:
- a primary vessel comprising an open end and a suspension comprising a target material;
- a processing vessel comprising
- a first end comprising a resealable plug,
- an inner cavity, and
- a displacement fluid comprising a density greater than a density of the target material, wherein the displacement fluid is located within the inner cavity;
- a device at least partially located within the open end of the primary vessel, the device comprising a cannula,
- wherein the cannula extends through the resealable plug of the processing vessel and accesses the inner cavity of the processing vessel, thereby mating the processing vessel and the device and fluidly connecting the primary vessel to the processing vessel,
- wherein the device extends upwardly from the open end of the primary vessel, and
- wherein the processing vessel extends upwardly from the device.
6 more elements
Dependencies are inferred from the claim text.