US 9,541,480 B2 · Capture, purification, and release of biological substances using a surface coating

Abstract

This invention relates to a surface coating for capture circulating rare cells, comprising a nonfouling composition to prevent the binding of non-specific cells and adsorption of serum components; a bioactive composition for binding the biological substance, such as circulating tumor cells; with or without a linker composition that binds the nonfouling and bioactive compositions. The invention also provide a surface coating for capture and purification of a biological substance, comprising a releasable composition to release the non-specific cells and other serum components; a bioactive composition for binding the biological substance, such as circulating tumor cells; with or without a linker composition that binds the releasable and bioactive compositions. The present invention also discloses a novel microfluidic chip, with specific patterned microstructures to create a flow disturbance and increase the capture rate of the biological substance.

Claims14

Dependent claims

A method comprising:

(a) contacting a biological sample comprising rare cells and non-specific cells to a non-fouling lipid layer of a microfluidic device, the microfluidic device comprising an antibody which selectively binds to rare cells, and wherein the non-fouling lipid layer is non-covalently associated with the antibody;
(b) binding the rare cells to the antibody;
(c) washing the non-fouling lipid layer with a liquid, wherein the non-specific cells in contact with the non-fouling lipid layer are released from the non-fouling lipid layer;
and
(d) flowing a liquid comprising bubbles through a channel of the microfluidic device of step (c), thereby releasing the rare cells from the microfluidic device.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

14/128,354Filed 28 Jun 2012 · us provisional application

Priority

29 Jun 2011

Published

US 2014/0255976 A111 Sep 2014

Granted

10 Jan 2017

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

G01N 33/54393
With an insoluble carrier for immobilising immunochemicals: improving reaction conditions or stability, e.g. by coating or irradiation of surface,…
C07K 16/30
Against receptors, cell surface antigens or cell surface determinants: from tumour cells
C07K 17/14
Carrier-bound or immobilised peptides ; Preparation thereof: peptides being immobilised on, or in, an inorganic carrier
G01N 1/405
Concentrating samples: by adsorption or absorption

Capture, purification, and release of biological substances using a surface coating

Life of this patent

Capture, purification, and release of biological substances using a surface coating

Life of this patent

Abstract

Claims14