Array fluorescence equalization method

A method for fluorescence intensity equalization comprising the following steps:

• providing an assay device having a plurality of immobilized arrays of molecular probes that bind to an analyte bound to a fluorescent marker;
• providing a drying apparatus comprising a plurality of aspiration tubes, each having open first and second ends, the second end of each aspiration tube being connected to a vacuum source for applying a vacuum through said tubes, said aspiration tubes each defining a length and defining a longitudinal bore along said length, said bore having a tapered diameter such that said first end has a larger diameter than said second end;
• placing the first end of each aspiration tube in proximity of a corresponding one of said plurality of immobilized arrays of molecular probes;
• and
• applying a vacuum in a range between about 106 decaPascals to about 10132 decaPascals through said aspiration tubes to generate vacuum induced air flow currents for removing vapor from said plurality of immobilized arrays of molecular probes, wherein sufficient vapor is removed to obtain a uniform relative dryness of said plurality of immobilized arrays of molecular probes within about 10 minutes.