PCA3 genes

An isolated DNA molecule which is:

• (a) a DNA molecule that comprises at least 30 consecutive nucleotides of:
• (i) SEQ ID NO:
• 1;
• (ii) SEQ ID NO:
• 3;
• or
• (iii) SEQ ID NO:
• 4,
• wherein said DNA molecule specifically hybridizes under high stringency conditions to a nucleic acid fully complementary to a PCA3 cDNA consisting of the nucleotide sequence of SEQ ID NO:
• 1, 3 or 4, and
• wherein said high stringency conditions comprise hybridization at 68° C. in 5×SSC, 5×Denhardt's solution, 1% SDS, and 100 μg/mL denatured carrier DNA, and washing at 68° C. in 0.2×SSC, 0.1% SDS;
• or
• (b) a DNA molecule fully complementary to (a),
• wherein said isolated DNA molecule comprises a detectable label which is a radiolabel, an enzyme, a fluorescent label, an affinity label, or a chemiluminescent label, wherein the detectable label is a non-nucleotide detectable label.

An isolated DNA molecule which is:

• (a) a DNA molecule comprising at least 15 consecutive nucleotides of:
• (i) SEQ ID NO:
• 1, which spans nucleotides 98 and 99, 263 and 264, 446 and 447, or 985 and 986 of SEQ ID NO:
• 1;
• (ii) SEQ ID NO:
• 3, which spans nucleotides 98 and 99, 281 and 282, or 820 and 821 of SEQ ID NO:
• 3;
• or
• (iii) SEQ ID NO:
• 4, which spans nucleotides 98 and 99 or 281 and 282 of SEQ ID NO:
• 4,
• wherein said DNA molecule specifically hybridizes under high stringency conditions to a nucleic acid fully complementary to a PCA3 cDNA consisting of the nucleotide sequence of SEQ ID NO:
• 1, 3 or 4, and
• wherein said high stringency conditions comprise hybridization at 68° C. in 5×SSC, 5×Denhardt's solution, 1% SDS, and 100 μg/mL denatured carrier DNA, and washing at 68° C. in 0.2×SSC, 0.1% SDS;
• or
• (b) a DNA molecule fully complementary to (a),
• wherein said isolated DNA molecule comprises a detectable label which is a radiolabel, an enzyme, a fluorescent label, an affinity label, or a chemiluminescent label, wherein the detectable label is a non-nucleotide detectable label.

An isolated DNA molecule which is:

• (1) a DNA molecule comprising:
• (a) the nucleotide sequence of PCA3 exon 1 having nucleotides 1-98 of SEQ ID NO:
• 1;
• (b) the nucleotide sequence of PCA3 exon 3 having nucleotides 264-446 of SEQ ID NO:
• 1;
• (c) the nucleotide sequence of PCA3 exon 4a having nucleotides 447-985 of SEQ ID NO:
• 1;
• or
• (d) the nucleotide sequence of PCA3 exon 4b having nucleotides 986-2037 of SEQ ID NO:
• 1;
• wherein said DNA molecule specifically hybridizes under high stringency conditions to a nucleic acid fully complementary to a PCA3 cDNA consisting of the nucleotide sequence of SEQ ID NO:
• 1, 3 or 4, and wherein said high stringency conditions comprise hybridization at 68° C. in 5×SSC, 5×Denhardt's solution, 1% SDS, and 100 μg/mL denatured carrier DNA, and washing at 68° C. in 0.2×SSC, 0.1% SDS;
• or
• (2) a DNA molecule fully complementary to any one of (1) (a) to (1) (d),
• wherein said isolated DNA molecule comprises a detectable label which is a radiolabel, an enzyme, a fluorescent label, an affinity label, or a chemiluminescent label, wherein the detectable label is a non-nucleotide detectable label.