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US 9,540,674 B2Granted
Methods of determining cellular chemosensitivity
AssigneeDana-Farber Cancer Institute, Inc.
InventorAnthony G. Letai
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Abstract
The present invention provides methods of determining cell sensitivity to a therapeutic agent.
Claims10
A method comprising:
- (a) providing a cancer cell sample;
- (b) permeabilizing a first aliquot of cells from the cancer cell sample;
- (c) contacting the first aliquot of permeabilized cells with a first purified BH3 domain peptide of 50 amino acids or less;
- (d) measuring the first BH3 domain peptide-induced mitochondrial outer membrane permeabilization (MOMP) in the first aliquot of cells;
- (e) permeabilizing a second aliquot of cells from the cancer cell sample;
- (f) contacting the second aliquot of permeabilized cells with a second purified BH3 domain peptide of 50 amino acids or less, wherein the first BH3 domain peptide and the second BH3 domain peptide are derived from different BCL-2 proteins;
- (g) measuring the second BH3 domain peptide-induced MOMP in the second aliquot of cells;
- and
- (h) determining a test BH3 profile for the cancer cell sample based on the first BH3 domain peptide-induced MOMP measured in (d) and the second BH3 domain peptide-induced MOMP measured in (g).
6 more elements
Dependencies are inferred from the claim text.