Methods for producing recombinant glycoproteins with modified glycosylation
AssigneeFountain Biopharma Inc.
InventorsNien-Yi Chen, Che-Haorz Wu, Hung-Chi Chen, Winston Town
Life of this patent
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Abstract
Genetically engineered host animal cells capable of producing glycoproteins having modified glycosylation patterns, e.g., defucosylation and/or monoglycosylation. Such host animal cells can be engineered to express fucosidase, endoglycosidase or both.
Claims12
A genetically engineered host animal cell, which expresses an exogenous fucosidase and an exogenous endoglycosidase, wherein the engineered host animal cell produces glycoproteins having modified glycosylation, wherein the exogenous fucosidase is bacterial fucosidase BF3242, wherein the exogenous endoglycosidase is an Endo S enzyme, and wherein the host animal cell is a Chinese hamster ovary (CHO) cell.
A method for producing a defucosylated glycoprotein, comprising:
- providing a genetically engineered host animal cell expressing (a) a glycoprotein, and (b) an exogenous fucosidase and (c) an exogenous endoglycosidase;
- culturing the host animal cell under conditions allowing for producing the glycoprotein, the fucosidase, and the endoglycosidase;
- and collecting the host animal cell or the culture supernatant for isolating the glycoprotein, wherein the exogenous fucosidase is bacterial fucosidase BF3242, wherein the exogenous endoglycosidase is an Endo S enzyme, and wherein the host animal cell is a Chinese hamster ovary (CHO) cell.
Dependencies are inferred from the claim text.