Method of producing glycolipids
AssigneeKao Corporation
InventorFumikazu Takahashi
Life of this patent
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Abstract
A protein selected from the following (a) to (c), a gene encoding the protein, a transformant in which the gene is subjected to deletion, mutation or repression of gene expression, and a method of producing a glycolipid using the transformant are provided, wherein: (a) is a protein consisting of an amino acid sequence set forth in SEQ ID NO: 1; (b) is a protein consisting of an amino acid sequence having 50% or more identity with the amino acid sequence set forth in SEQ ID NO: 1, and having alcohol oxidase activity; and (c) is a protein consisting of an amino acid sequence in which one to several amino acids are subjected to deletion, substitution, insertion or addition in the amino acid sequence set forth in SEQ ID NO: 1, and having alcohol oxidase activity.
Claims18
A transformant of a host, the host having a gene encoding any one of the following proteins (a) to (c),
- wherein, in the transformant, the gene is deleted, mutated or subject to repression of gene expression, and
- wherein, in the transformant, as a result of the gene being deleted, mutated or subject to repression of gene expression, alcohol oxidase activity is decreased as compared to that in the host,
- wherein, proteins (a) to (c) are:
- (a) A protein consisting of an amino acid sequence set forth in SEQ ID NO:
- 1;
- (b) A protein consisting of an amino acid sequence having 90% or more identity with the amino acid sequence set forth in SEQ ID NO:
- 1, and having alcohol oxidase activity;
- and
- (c) A protein consisting of an amino acid sequence in which one to ten amino acids in the amino acid sequence set forth in SEQ ID NO:
- 1 have been deleted, substituted, inserted or added, and having alcohol oxidase activity.
Dependencies are inferred from the claim text.