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US 9,540,671 B2Granted
Capping prone RNA polymerase enzymes and their applications
AssigneeEukarys
InventorPhilippe Jais
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Abstract
The invention provides a chimeric enzyme comprising at least one catalytic domain of a RNA triphosphatase, at least one catalytic domain of a guanylyltransferase, at least one catalytic domain of a N7-guanine methyltransferase, and at least one catalytic domain of a DNA-dependant RNA polymerase. The invention also provides pharmaceutical composition comprising said chimeric enzyme and uses of said chimeric enzyme.
Claims13
A hetero-oligomeric enzyme comprising:
- a catalytic domain of a RNA triphosphatase;
- a catalytic domain of a guanylyltransferase;
- a catalytic domain of a N7-guanine methyltransferase;
- and
- a catalytic domain of a DNA-dependent RNA polymerase,
- wherein the catalytic domain of a DNA-dependent RNA polymerase is linked covalently or non-covalently with the catalytic domain of a RNA triphosphatase, the catalytic domain of a guanylyltransferase, or the catalytic domain of a N7-guanine methyltransferase, and
- wherein the hetero-oligomeric enzyme is non-natural.
4 more elements
A hetero-oligomeric enzyme comprising:
- a monomer comprising a catalytic domain of a RNA triphosphatase, a catalytic domain of a guanylyltransferase, and a catalytic domain of a N7-guanine methyltransferase;
- and
- a complex comprising a catalytic domain of a DNA-dependent RNA polymerase,
- wherein the monomer and the complex are linked covalently or non-covalently, and
- wherein the hetero-oligomeric enzyme is non-natural.
2 more elements
Dependencies are inferred from the claim text.