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US 9,540,669 B2Granted
Trichoderma reesei glucoamylase variants resistant to oxidation-related activity loss and the use thereof
AssigneeDanisco US Inc.
InventorsSu Yin Marina Chow, Thomas P. Graycar, Jacquelyn A. Huitink, Casper Vroemen, David L. Wong
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Abstract
Embodiments of the present disclosure relate to Trichoderma reesei glucoamylase (TrGA) variants having improved properties (e.g., improved thermostability, improved specific activity, and/or resistant to oxidation-related activity loss). Also provided are compositions comprising variant glucoamylases. These compositions are useful in various starch process applications.
Claims24
A glucoamylase variant comprising amino acid substitutions corresponding to positions:
- 50, 417, 430, 511, 539, and 563 of SEQ ID NO:
- 2, wherein the glucoamylase variant has at least 90% sequence identity with SEQ ID NO:
- 2, and wherein the amino acid substitution at position 50 is M50Y, G, F, or K.
Dependencies are inferred from the claim text.