Nucleic acid fragment binding to target protein
AssigneeTagcyx Biotechnologies
InventorsIchiro Hirao, Michiko Hirao, Rie Yamashige, Shigeyuki Yokoyama
Life of this patent
Estimating the term…
Abstract
An object of the present invention is to develop and provide a method for efficiently producing a nucleic acid aptamer, particularly, a DNA aptamer, having higher specificity and binding activity against a target substance than those of nucleic acid aptamers obtained by conventional methods. The present invention provides a transcribable or replicable nucleic acid aptamer comprising a natural nucleotide and a non-natural nucleotide having an artificial base-pairable artificial base. The present invention also provides a method for sequencing a non-natural nucleotide-containing single-stranded nucleic acid molecule selected from a single-stranded nucleic acid library.
Claims7
A transcribable or replicable nucleic acid aptamer comprising:
- natural nucleotides and
- non-natural nucleotides having an artificial base-pairable artificial base,
- wherein the artificial base is selected from the group consisting of 7-(2-thienyl)-3H-imidazo[4,5-b]pyridin-3-yl, 2-nitropyrrol-1-yl, and 2-formyl-1H-pyrrol-1-yl, and wherein the nucleic acid aptamer is directed against interferon γ as a target substance,
- wherein the nucleic acid aptamer comprises any one nucleotide sequence selected from the group consisting of SEQ ID NOs:
- 167 to 174 (provided that “n” in the sequences represents 7-(2-thienyl)-3H-imidazo[4,5-b]pyridin-3-yl), 186, 188, 190, 192, 194, 214 to 222, and 279 to 328.
Dependencies are inferred from the claim text.