Method and materials for isolation of nucleic acid materials

A method for nucleic acid extraction, the method comprising:

• receiving a binding moiety solution within a process chamber, wherein the binding moiety solution comprises a collection buffer and a set of affinity moiety-coated microparticles;
• mixing the binding moiety solution with a biological sample, within the process chamber, thereby producing a moiety-sample mixture;
• incubating the moiety-sample mixture to reversibly bind nucleic acid material, comprising target DNA and target RNA, of the biological sample to the set of affinity moiety-coated microparticles, thereby producing a set of moiety-bound nucleic acid particles;
• receiving a cartridge at a cartridge receiving module comprising a set of pins, each pin in the set of pins displaceable between a first position and a second position;
• occluding a fluidic pathway of the cartridge upon displacement of a first subset of the set of pins;
• and
• magnetically separating the set of moiety-bound nucleic acid particles from the moiety-sample mixture, within the fluidic pathway of the cartridge.

A method for nucleic acid isolation from a biological sample, comprising:

• receiving a binding moiety solution within a process chamber, wherein the binding moiety solution includes a set of affinity moiety-coated microparticles;
• producing a moiety-sample mixture upon combination of the binding moiety solution with the biological sample;
• producing a set of moiety-bound nucleic acid particles upon incubation of the moiety-sample mixture to reversibly bind nucleic acid material of the biological sample to the set of affinity moiety-coated microparticles;
• occluding a fluidic pathway of a cartridge upon displacement of a first subset of a set of pins at the cartridge comprising the set of pins;
• separating the set of moiety-bound nucleic acid particles from the moiety-sample mixture, within the fluidic pathway of the cartridge;
• washing the set of moiety-bound nucleic acid particles, within the fluidic pathway of the cartridge;
• and
• releasing a nucleic acid sample from the set of moiety-bound nucleic acid particles with an elution solution.