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US 9,540,633 B2Granted
High expression cereal phytase gene
AssigneeAarhus Universitet
InventorsHenrik Brinch-Pedersen, Claus K Madsen, Giuseppe Dionisio, Preben Bach Holm
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Abstract
The present invention provides mutant cereal plants and mature grain thereof, characterised by enhanced levels of the enzyme phytase in the grain, and methods for inducing, detecting and selecting the mutant cereal plants. The invention further relates to animal feed comprising said grain having enhanced amounts of phytase.
Claims4
A method for producing a Triticum plant capable of producing an average phytase endosperm content of greater than 4300 FTU/kg, said method comprising:
- a) obtaining a sample of nucleic acids from a Triticum plant or portion thereof;
- b) detecting in said sample the presence of the nucleotide V at the 5′ end of a polynucleotide having the nucleotide sequence ACA VGA GTC ATG CAT (SEQ ID NO:1), wherein V is a cytosine;
- c) breeding a Triticum plant comprising said nucleotide sequence with a second Triticum plant to obtain grains;
- and
- d) growing at least one Triticum plant from said grains;
- wherein said Triticum plant grown from said grains comprises said nucleotide V at the 5′ end of a polynucleotide having the nucleotide sequence ACA VGA GTC ATG CAT (SEQ ID NO:
- 1), and wherein said V is a cytosine.
4 more elements
Dependencies are inferred from the claim text.