Human carbonic anhydrase II with increased physical stability
AssigneeInzymes Biotech AB
InventorsUno Carlsson, Martin Karlsson
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Abstract
An isolated polypeptide having carbonic anhydrase activity, the sequence of which corresponds to modified human carbonic anhydrase II is described. The isolated polypeptide comprises the mutations A23C, S99C, L202C, C205S and V241C and the polypeptide has increased physical stability compared to wild type carbonic anhydrase II. Further, the polypeptide comprises disulfide bridges between C23 and C202 and/or between C99 and C241.
Claims10
An isolated polypeptide having carbonic anhydrase activity, the sequence of which corresponds to modified human carbonic anhydrase II, wherein the polypeptide comprises the mutations A23C, S99C, L202C, C205S and V241C relative to wild type human carbonic anhydrase II having the amino acid sequence of SEQ ID NO:9, has increased physical stability compared to wild type carbonic anhydrase II and further comprises disulfide bridges between C23 and C202 and/or between C99 and C241;
- and
- wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:
- 8.
Dependencies are inferred from the claim text.