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US 9,540,622 B2Granted
Engineered biocatalysts useful for carbapenem synthesis
AssigneeCodexis, Inc.
InventorsJoly Sukumaran, Derek Smith, Hong Yang, Wan Lin Yeo, Jeffrey C. Moore
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Abstract
The present disclosure provides engineered pNB esterase polypeptides useful for the synthesis of the carbapenem antibiotic, imipenem. The disclosure also provides polynucleotides encoding the engineered pNB esterases, host cells capable of expressing the engineered pNB esterases, and methods of using the engineered pNB esterases in the production of imipenem.
Claims32
An engineered polypeptide having p-nitrobenzyl esterase (pNB) activity, comprising an amino acid sequence having at least 90% identity to SEQ ID NO:
- 2 and one or more residue differences as compared to SEQ ID NO:
- 2 at residue positions selected from:
- 108, 115, 116, 130, 193, 214, 219, 273, 276 and 321, wherein the differences at positions 115 and 273 are 115Q/W and 273A/E/T/V, respectively, and optionally wherein said engineered polypeptide further comprises a different residue at position 362.
Dependencies are inferred from the claim text.