Method for producing horseradish peroxidase recombinant protein using filamentous fungus

A polynucleotide modified to have at least one codon whose base sequence is different from a wild-type base sequence encoding a horseradish peroxidase polypeptide, having codon usage frequencies in the following percentages, and being capable of expressing the polypeptide to be encoded in a filamentous fungus, wherein

• in a case where an amino acid encoded by the modified codon is alanine, a usage frequency of GCC is 80% and a usage frequency of GCT is 20%;
• in a case where the amino acid encoded by the modified codon is arginine, a usage frequency of CGC is 90% and a usage frequency of CGT is 10%;
• in a case where the amino acid encoded by the modified codon is asparagine, a usage frequency of AAC is 100%;
• in a case where the amino acid encoded by the modified codon is aspartic acid, a usage frequency of GAC is 95% and a usage frequency of GAT is 5%;
• in a case where the amino acid encoded by the modified codon is cysteine, a usage frequency of TGC is 100%;
• in a case where the amino acid encoded by the modified codon is glutamine, a usage frequency of CAG is 100%;
• in a case where the amino acid encoded by the modified codon is glutamic acid, a usage frequency of GAG is 100%;
• in a case where the amino acid encoded by the modified codon is glycine, a usage frequency of GGC is 75% and a usage frequency of GGT is 25%;
• in a case where the amino acid encoded by the modified codon is histidine, a usage frequency of CAC is 100%;
• in a case where the amino acid encoded by the modified codon is isoleucine, a usage frequency of ATC is 100%;
• in a case where the amino acid encoded by the modified codon is leucine, a usage frequency of CTC is 80% and a usage frequency of CTG is 20%;
• in a case where the amino acid encoded by the modified codon is lysine, a usage frequency of AAG is 100%;
• in a case where the amino acid encoded by the modified codon is phenylalanine, a usage frequency of TTC is 100%;
• in a case where the amino acid encoded by the modified codon is proline, a usage frequency of CCC is 80% and a usage frequency of CCT is 20%;
• in a case where the amino acid encoded by the modified codon is serine, a usage frequency of AGC is 15% and a usage frequency of TCC is 85%;
• in a case where the amino acid encoded by the modified codon is threonine, a usage frequency of ACC is 85% and a usage frequency of ACG is 15%;
• in a case where the amino acid encoded by the modified codon is tyrosine, a usage frequency of TAC is 100%;
• and
• in a case where the amino acid encoded by the modified codon is valine, a usage frequency of GTC is 85%, a usage frequency of GTG is 5%, and a usage frequency of GTT is 10% wherein said polynucleotide encodes a horseradish peroxidase C1a polypeptide and has at least one characteristic selected from the group consisting of the following (i) to (iv):
• (i) comprising a coding region of a base sequence of SEQ ID NO:
• 1;
• (ii) having a sequence identity of 95% or more with a base sequence at positions 91 to 1017 of SEQ ID NO:
• 1;
• (iii) comprising a coding region of a base sequence of SEQ ID NO:
• 26;
• and
• (iv) having a sequence identity of 95% or more with a base sequence at positions 91 to 1017 of SEQ ID NO:
• 26.