Back to results
US 9,540,615 B2Granted
Combined chemical and genetic approaches for generation of induced pluripotent stem cells
AssigneeThe Scripps Research Institute
InventorsYan Shi, Caroline Desponts, Sheng Ding, Hongyan Zhou, Tongxiang Lin, Wenlin Li, Saiyong Zhu
Life of this patent
Estimating the term…
Abstract
The present invention provides for identification and use of small molecules to induce pluripotency in mammalian cells as well as other methods of inducing pluripotency.
Claims9
An in vitro method of producing an induced pluripotent stem cell from a mammalian non-pluripotent cell, the method comprising:
- (a) contacting the mammalian non-pluripotent cell with a MEK inhibitor;
- and
- (b) introducing into the mammalian non-pluripotent cell, one or more expression cassettes comprising nucleic acids encoding an Oct4 polypeptide and a Klf4 polypeptide, thereby producing an induced pluripotent stem cell;
- wherein the introducing step does not comprise introducing a Myc polypeptide or introducing an expression cassette comprising a nucleic acid encoding a Myc polypeptide.
1 more element
A composition comprising a mammalian non-pluripotent cell;
- one or more expression cassettes comprising nucleic acids encoding a Klf4 polypeptide, an Oct4 polypeptide, and/or a Sox2 polypeptide;
- and at least one of a TGFβ receptor/ALK5 inhibitor, a GSK3 inhibitor, and a MEK inhibitor, wherein the one or more expression cassettes does not encode a Myc polypeptide.
Dependencies are inferred from the claim text.