Method for generating cancer stem cells from immortalized cell lines
AssigneeKorea University Research and Business Foundation
InventorsSunghoi Hong, Seung-Ick Oh, Hang-Soo Park
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Abstract
A method is described for generating a novel cancer stem cell line that possesses characteristics associated with stem cells, by co-culturing a human immortalized cell line and bone marrow-derived mesenchymal stem cells, and the novel cancer stem cell line established thereby. This method is able to readily generate cancer stem cells that are low in the level of structural chromosomal aberrations and are excellent in oncogenicity, and is effectively applicable to the development of anti-cancer drugs and personalized drugs.
Claims10
A method for generating a cancer stem cell line from an immortalized 293FT cell line, comprising:
- co-culturing an immortalized 293FT cell line and mesenchymal stem cells in an induction medium, with the mesenchymal stem cells serving as feeder cells, for sufficient time to generate cancer stem cells characterized by neural stem cell markers CD15+ and CD 133+, and sub-culturing same to produce CD15+ and CD 133+ cells as said cancer stem cell line,
- wherein the induction medium comprises FBS, L-glutamine, L-alanyl-glutamine and high-glucose DMEM.
Dependencies are inferred from the claim text.