Fill system for providing uniform concentrations and volumes and methods thereof

A closed and sterile system comprising

• a starting container;
• a system of fluid lines having a plurality of fluid lines;
• a plurality of containers;
• a fluid flow regulator
• at least one multi-connector
• a connecting fluid line which connects the starting container with the system of fluid lines;
• and
• a syringe connected to the connecting fluid line, wherein the connector for connecting the syringe to the fluid line allows for pushing fluid or gas into and removing fluid or gas out of the connecting fluid line through the syringe connector;
• wherein each container of the plurality of containers is connected to a fluid line of the plurality of fluid lines so as to form a closed system in which a fluid may flow from the starting container through the system of fluid lines to the plurality of containers;
• and
• wherein the plurality of containers are each independently removably attached to each other via the system fluid lines;
• and
• wherein the plurality of fluid lines are arranged in uniform and parallel fashion and are each engaged with one channel of the multichannel peristaltic pump;
• and
• wherein the plurality of fluid lines have fittings which can be used to secure the plurality of fluid lines in a pump head of a multichannel peristaltic pump;
• wherein the at least one multi-connector and fluid flow regulator are upstream of the multichannel peristaltic pump;
• and
• wherein the uniform and parallel arrangement of the fluid lines and placement of the at least one multi-connector and a syringe upstream of the multichannel peristaltic pump of the closed and sterile system divides and dispenses uniform concentrations and amounts of a suspension into the plurality of containers from the starting container when the multichannel peristaltic pump is operated in the forward direction.